catalog number :
MBS035286
products type :
ELISA Kit
products full name :
Mouse Heat Shock 70Kda Protein 4 ELISA Kit
products short name :
Heat Shock 70Kda Protein 4
other names :
heat shock 70 kDa protein 4; Heat shock 70 kDa protein 4; heat shock 70 kDa protein 4; hsp70 RY; heat shock 70kD protein 4; heat shock protein, 110 kDa; heat shock 70-related protein APG-2; heat shock 70kDa protein 4; HSP70RY; Heat shock 70-related protein APG-2
products gene name :
HSPA4
other gene names :
HSPA4; HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HS24/P52; APG2
uniprot entry name :
HSP74_HUMAN
specificity :
No significant cross-reactivity or interference between Mouse HSPA4 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HSPA4 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse HSPA4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HSPA4 and HSPA4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HSPA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HSPA4 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse HSPA4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HSPA4 and HSPA4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HSPA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_002145.3
ncbi gb acc num :
NM_002154.3
ncbi mol weight :
94,331 Da
ncbi pathways :
Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Parkin-Ubiquitin Proteasomal System Pathway (700638); Validated Targets Of C-MYC Transcriptional Activation Pathway (169351)
uniprot summary :
HSP70RY: Interacts with TJP1/ZO-1. Belongs to the heat shock protein 70 family. Protein type: Chaperone; Heat shock protein. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: mitochondrion; cytosol. Molecular Function: ATP binding. Biological Process: chaperone-mediated protein complex assembly; protein import into mitochondrial outer membrane; response to unfolded protein