catalog number :
MBS035254
products type :
ELISA Kit
products full name :
Human Nitrotyrosine ELISA Kit
products short name :
Nitrotyrosine
other names :
nitric oxide synthase, inducible; Nitric oxide synthase, inducible; nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 2, inducible; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2
other gene names :
NOS2; NOS2; iNOS; NOS2A; Inducible NOS; iNOS; NOSII
uniprot entry name :
NOS2_BOVIN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 0.25 nmol/L - 8 nmol/L. Sensitivity: 0.1 nmol/L.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of NT (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_001070267.1
ncbi gb acc num :
NM_001076799.1
ncbi mol weight :
131,208 Da
ncbi pathways :
Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (84202); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (84289); Calcium Signaling Pathway (459); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Disease Pathway (942846); HIF-1 Signaling Pathway (695207)
uniprot summary :
Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 . By similarity. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group . By similarity.Binds 1 FAD . By similarity.Binds 1 FMN . By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme . By similarity. Enzyme regulation: Regulated by calcium/calmodulin . By similarity. Subunit structure: Homodimer. Binds SLC9A3R1 . By similarity. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.