ELISA/assay

Hamster Heme Oxygenase 1, Decycling ELISA Kit

MBS034959

96 Strip Wells

600 USD

ELISA Kit

Hamster Heme Oxygenase 1, Decycling ELISA Kit

Heme Oxygenase 1, Decycling

heme oxygenase 1; Heme oxygenase 1; heme oxygenase 1; heat shock protein, 32-kD; heme oxygenase (decycling) 1

HO1

HMOX1; HMOX1; HO-1; HSP32; HMOX1D; bK286B10; HO; HO1; HO-1

HMOX1_HUMAN

Hamster

No significant cross-reactivity or interference between Hamster HO1 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HO1 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster HO1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HO1 and HO1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 0.625ng/ml-20ng/ml.

Sensitivity: The sensitivity of this kit is 0.1ng/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HO1 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster HO1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HO1 and HO1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4504437

NP_002124.1

NM_002133.2

P09601

32,819 Da

HIF-1 Signaling Pathway 695200!!HIF-1-alpha Transcription Factor Network Pathway 138045!!Heme Degradation Pathway 160971!!Iron Uptake And Transport Pathway 187191!!Keap1-Nrf2 Pathway 198777!!Metabolism Pathway 477135!!Metabolism Of Porphyrins Pathway 106325!!Mineral Absorption Pathway 212237!!Mineral Absorption Pathway 212220!!Oxidative Stress Pathway 198916

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]

Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Catalytic activity: Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. Subcellular location: Microsome. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side Ref.12. Tissue specificity: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). Ref.9. Induction: Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Involvement in disease: Heme oxygenase 1 deficiency (HMOX1D) [MIM:614034]: A disease characterized by impaired stress hematopoiesis, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues. Clinical features include persistent hemolytic anemia, asplenia, nephritis, generalized erythematous rash, growth retardation and hepatomegaly.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7. Sequence similarities: Belongs to the heme oxygenase family.

96 Strip Wells

600 USD