catalog number :
MBS034847
products type :
ELISA Kit
products full name :
Rat Phosphoenolpyruvate Carboxykinase ELISA Kit
products short name :
Phosphoenolpyruvate Carboxykinase
other names :
phosphoenolpyruvate carboxykinase; phosphoenolpyruvate carboxykinase
other gene names :
Niako_5471
specificity :
No significant cross-reactivity or interference between Rat PCK and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 U/L - 100 U/L. Sensitivity: 1.0 U/L.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCK concentrations in Rat serum, plasma and other body fluids. Using Purified Rat PCK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCK and PCK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCK in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCK concentrations in Rat serum, plasma and other body fluids. Using Purified Rat PCK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCK and PCK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
YP_005011109.1
ncbi gb acc num :
NC_016609.1
ncbi pathways :
Biosynthesis Of Secondary Metabolites Pathway (548361); Citrate Cycle (TCA Cycle) Pathway (548283); Citrate Cycle (TCA Cycle) Pathway (288); Glycolysis / Gluconeogenesis Pathway (548282); Glycolysis / Gluconeogenesis Pathway (287); Microbial Metabolism In Diverse Environments Pathway (548364); Pyruvate Metabolism Pathway (548380); Pyruvate Metabolism Pathway (373)