ELISA/assay

Mouse Defensin 5 ELISA Kit

MBS034540

48-Strip-Wells

435 USD

ELISA Kit

Mouse Defensin 5 ELISA Kit

Defensin 5

defensin-5 preproprotein; Defensin-5; defensin-5; HD5(20-94); defensin 5; defensin, alpha 5, preproprotein; defensin, alpha 5, Paneth cell-specific; Defensin, alpha 5; HD5(20-94)

DEF5

DEFA5; DEFA5; DEF5; HD-5; DEF5

DEF5_HUMAN

Mouse

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Cell Culture Supernatants, Tissue Homogenate, Urine and other Body Fluids. Detection Range: 25 pg/ml ~ 800 pg/ml.

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Mouse DEF5 in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Mouse DEF5 concentration. The concentration of Mouse DEF5 in the specimens is then determined by comparing the O.D. of the specimens to the standard curve. Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Mouse DEF5 in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Mouse DEF5 concentration. The concentration of Mouse DEF5 in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.

Background: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of specimens. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the specimen.

10337585

NP_066290.1

NM_021010.1

Q01523

10,071 Da

Alpha-defensins Pathway (530758); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Jul 2008]

DEFA5: Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. Belongs to the alpha-defensin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular space; transport vesicle; Golgi lumen; extracellular region. Biological Process: killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus

48-Strip-Wells

435 USD

96-Strip-Wells

600