ELISA/assay

Canine Indoleamine 2,3-Dioxygenase ELISA Kit

MBS034272

48-Strip-Wells

435 USD

ELISA Kit

Canine Indoleamine 2,3-Dioxygenase ELISA Kit

Indoleamine 2,3-Dioxygenase

Indoleamine 2,3-dioxygenase; Indoleamine 2,3-dioxygenase; Bna2p; Biosynthesis of nicotinic acid protein 2

IDO

BNA2; BNA2; IDO

I23O_YEAST

Canine

No significant cross-reactivity or interference between Canine IDO and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IDO concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IDO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IDO and IDO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IDO in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IDO concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IDO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IDO and IDO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IDO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1176339

P47125.1

P47125

50,775 Da

De Novo NAD Biosynthesis Pathway (198675); Metabolism Pathway (829779); Metabolism Of Amino Acids And Derivatives Pathway (829897); NAD Biosynthesis II (from Tryptophan) Pathway (143526); NAD Biosynthesis II (from Tryptophan) Pathway (138368); Tryptophan Degradation Via Kynurenine Pathway (198749); Tryptophan Catabolism Pathway (829904); Tryptophan Metabolism Pathway (889); Tryptophan Metabolism Pathway (332); Superpathway Of NAD Biosynthesis In Eukaryotes (138369)

Function: Catalyzes the first step in tryptophan catabolism in order to supply de novo nicotinamide adenine dinucleotide (NAD+) via the kynurenine pathway. Plays a role in the cellular response to telomere uncapping. Catalytic activity: D-tryptophan + O2 = N-formyl-D-kynurenine.L-tryptophan + O2 = N-formyl-L-kynurenine. Cofactor: Binds 1 heme group per subunit . By similarity. Pathway: Cofactor biosynthesis; NAD(+) biosynthesis. Ref.4. Induction: Expression is mediated by the AFT2, HCM1, and SUM1 transcription factors. Up-regulated in absence of NPT1. Miscellaneous: Present with 149 molecules/cell in log phase SD medium. Sequence similarities: Belongs to the indoleamine 2,3-dioxygenase family. Biophysicochemical propertiesKinetic parameters:KM=412 uM for L-tryptophanKM=9.2 mM for D-tryptophan

48-Strip-Wells

435 USD

96-Strip-Wells

600