Chicken Ferroportin ELISA Kit | MyBioSource MBS034263 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Chicken Ferroportin ELISA Kit

catalog :

MBS034263

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS034263

products type :

ELISA Kit

products full name :

Chicken Ferroportin ELISA Kit

products short name :

Ferroportin

other names :

ferroportin 1 variant I, partial; Solute carrier family 40 member 1; solute carrier family 40 member 1; iron regulated gene 1; putative ferroportin 1 variant IIIA; putative ferroportin 1 variant IIIB; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3; solute carrier family 40 (iron-regulated transporter), member 1; Ferroportin-1; Iron-regulated transporter 1

products gene name :

FPN

other gene names :

SLC40A1; SLC40A1; FPN1; HFE4; MTP1; IREG1; MST079; MSTP079; SLC11A3; FPN1; IREG1; SLC11A3

uniprot entry name :

S40A1_HUMAN

reactivity :

Chicken

sequence length :

specificity :

No significant cross-reactivity or interference between Chicken FPN and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 ng/ml - 500 ng/ml. Sensitivity: 2.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FPN concentrations in Chicken serum, plasma and other body fluids. Using Purified Chicken FPN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FPN and FPN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FPN in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FPN concentrations in Chicken serum, plasma and other body fluids. Using Purified Chicken FPN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FPN and FPN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FPN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

67633296

ncbi acc num :

AAY78556.1

uniprot acc num :

Q9NP59

ncbi mol weight :

62,542 Da

ncbi pathways :

Iron Metabolism In Placenta Pathway (672461); Iron Uptake And Transport Pathway (187191); Metal Ion SLC Transporters Pathway (161066); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)

ncbi summary :

The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4). [provided by RefSeq, Jul 2008]

uniprot summary :

SLC40A1: May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin). Defects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4). HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation. Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily. Protein type: Transporter, SLC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 2q32. Cellular Component: multivesicular body; synaptic vesicle; basolateral plasma membrane; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; intracellular. Molecular Function: protein binding; iron ion transmembrane transporter activity. Biological Process: anatomical structure morphogenesis; cellular iron ion homeostasis; positive regulation of transcription from RNA polymerase II promoter; lymphocyte homeostasis; transmembrane transport; negative regulation of apoptosis. Disease: Hemochromatosis, Type 4

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage