ELISA/assay

Human Proliferating Cell Nuclear Antigen ELISA Kit

MBS034032

48-Strip-Wells

435 USD

ELISA Kit

Human Proliferating Cell Nuclear Antigen ELISA Kit

Proliferating Cell Nuclear Antigen

PCNA; Proliferating cell nuclear antigen; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein; proliferating cell nuclear antigen

PCNA

PCNA; PCNA

Q6FHF5_HUMAN

Human

No significant cross-reactivity or interference between Human PCNA and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCNA concentrations in Human serum, plasma and other body fluids. Using Purified Human PCNA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCNA and PCNA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCNA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCNA concentrations in Human serum, plasma and other body fluids. Using Purified Human PCNA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCNA and PCNA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCNA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

49456555

CAG46598.1

Q6FHF5

28,706 Da

BARD1 Signaling Events Pathway (137959); BRCA1-associated Genome Surveillance Complex (BASC) Pathway (413428); Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3 - 5 exonuclease and 3 -phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family. Protein type: DNA replication; Cell cycle regulation. Chromosomal Location of Human Ortholog: 20pter-p12. Cellular Component: nucleoplasm; centrosome; PCNA complex; cytoplasm; DNA replication factor C complex; nuclear replication fork; nucleus. Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; purine-specific mismatch base pair DNA N-glycosylase activity; MutLalpha complex binding; receptor tyrosine kinase binding; dinucleotide insertion or deletion binding. Biological Process: mismatch repair; heart development; positive regulation of deoxyribonuclease activity; DNA strand elongation during DNA replication; response to lipid; DNA repair; leading strand elongation; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; regulation of DNA replication; G1/S transition of mitotic cell cycle. Disease: Ataxia-telangiectasia-like Disorder 2

48-Strip-Wells

435 USD

96-Strip-Wells

600