Canine Complement Component 3B ELISA Kit | MyBioSource MBS033964 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Complement Component 3B ELISA Kit

catalog :

MBS033964

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS033964

products type :

ELISA Kit

products full name :

Canine Complement Component 3B ELISA Kit

products short name :

Complement Component 3B

other names :

complement receptor type 1 isoform S; Complement receptor type 1; complement receptor type 1; CD35 antigen; C3b/C4b receptor; C3-binding protein; Knops blood group antigen; complement component (3b/4b) receptor 1 (Knops blood group); C3b/C4b receptor

products gene name :

C3B

other gene names :

CR1; CR1; KN; C3BR; C4BR; CD35; C3BR

uniprot entry name :

CR1_HUMAN

reactivity :

Canine

sequence length :

2489

specificity :

No significant cross-reactivity or interference between Canine C3B and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 mug/ml - 1000 mug/ml. Sensitivity: 5.0 mug/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated C3B concentrations in Canine serum, plasma and other body fluids. Using Purified Canine C3B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add C3B and C3B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C3B in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated C3B concentrations in Canine serum, plasma and other body fluids. Using Purified Canine C3B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add C3B and C3B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

86793109

ncbi acc num :

NP_000642.3

ncbi gb acc num :

NM_000651.4

uniprot acc num :

P17927

ncbi mol weight :

223,663 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (106386); Innate Immune System Pathway (106387); Legionellosis Pathway (469200); Legionellosis Pathway (469186)

ncbi summary :

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

CR1: Mediates cellular binding of particles and immune complexes that have activated complement. Belongs to the receptors of complement activation (RCA) family. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: cell surface; integral to plasma membrane; plasma membrane. Molecular Function: complement component C3b receptor activity; complement component C4b binding; complement component C4b receptor activity; complement component C3b binding. Biological Process: negative regulation of complement activation, alternative pathway; complement receptor mediated signaling pathway; negative regulation of complement activation, classical pathway; regulation of complement activation; innate immune response; complement activation, classical pathway. Disease: Knops Blood Group System; Malaria, Susceptibility To

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage