ELISA/assay

Porcine Phospho-Exiracellular Signal-Regulated Kinase ELISA Kit

MBS033952

96 Strip Wells

600 USD

ELISA Kit

Porcine Phospho-Exiracellular Signal-Regulated Kinase ELISA Kit

Phospho-Exiracellular Signal-Regulated Kinase

protein phosphatase 1A isoform 3; Protein phosphatase 1A; protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA

PERK

PPM1A; PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA; PPPM1A; PP2C-alpha

PPM1A_HUMAN

Porcine

455

No significant cross-reactivity or interference between Porcine PERK and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PERK concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine PERK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PERK and PERK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PERK in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 62.5 pg/ml - 2000 pg/ml.

Sensitivity: The sensitivity of this kit is 10 pg/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

193211600

NP_808821.2

NM_177952.2

P35813

42,448 Da

BMP Receptor Signaling Pathway 137948!!Disease Pathway 530764!!Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway 645266!!Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway 160972!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!IGF1R Signaling Cascade Pathway 730346!!IRS-mediated Signalling Pathway 106426!!IRS-related Events Pathway 106424!!IRS-related Events Triggered By IGF1R Pathway 730348

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Function: Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB. Ref.8 Ref.9. Catalytic activity: [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate. Cofactor: Binds 2 magnesium or manganese ions per subunit. Subunit structure: Monomer. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with the phosphorylated form of IKBKB/IKKB. Ref.8 Ref.9. Subcellular location: Nucleus. Cytoplasm cytosol. Membrane . By similarity. Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization . By similarity. Ref.8 Ref.13. Post-translational modification: N-myristoylation is essential for the recognition of its substrates for dephosphorylation . By similarity. Sequence similarities: Belongs to the PP2C family.

96 Strip Wells

600 USD