Mouse Alpha-Fetoprotein ELISA Kit | MyBioSource MBS033826 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Alpha-Fetoprotein ELISA Kit

catalog :

MBS033826

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS033826

products type :

ELISA Kit

products full name :

Mouse Alpha-Fetoprotein ELISA Kit

products short name :

Alpha-Fetoprotein

other names :

alpha-fetoprotein; Alpha-fetoprotein; alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein; alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

products gene name :

AFP

other gene names :

AFP; AFP; FETA; HPAFP; HPAFP

uniprot entry name :

FETA_HUMAN

reactivity :

Mouse

specificity :

No significant cross-reactivity or interference between Mouse AFP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated AFP concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse AFP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AFP and AFP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AFP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated AFP concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse AFP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AFP and AFP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AFP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4501989

ncbi acc num :

NP_001125.1

ncbi gb acc num :

NM_001134.1

uniprot acc num :

P02771

ncbi mol weight :

68,678 Da

ncbi pathways :

Direct P53 Effectors Pathway (137939); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

ncbi summary :

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

uniprot summary :

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; cytoplasm. Molecular Function: metal ion binding. Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle. Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage