catalog number :
MBS033595
products type :
ELISA Kit
products full name :
Human Carboxymethyl Lysine ELISA Kit
products short name :
Carboxymethyl Lysine
other names :
Cysteine synthase A; cysteine synthase A, O-acetylserine sulfhydrolase A subunit
specificity :
No significant cross-reactivity or interference between Human CML and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 ng/ml - 500 ng/ml. Sensitivity: 2.0 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CML concentrations in Human serum, plasma and other body fluids. Using Purified Human CML antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CML and CML antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CML in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CML concentrations in Human serum, plasma and other body fluids. Using Purified Human CML antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CML and CML antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CML in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi pathways :
Biosynthesis Of Amino Acids Pathway (790553); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (815454); Carbon Metabolism Pathway (817567); Cysteine And Methionine Metabolism Pathway (103490); Cysteine And Methionine Metabolism Pathway (103421); Cysteine Biosynthesis, Serine = Cysteine Pathway (404603); Cysteine Biosynthesis, Serine = Cysteine Pathway (468214); Microbial Metabolism In Diverse Environments Pathway (179843); Sulfur Metabolism Pathway (6834)
ncbi summary :
CysK forms a bienzyme complex with CysE. [More information is available at EcoGene: EG10192]. Cysteine synthase A carries out the second step in the pathway of cysteine biosynthesis, converting O-acetyl-serine into L-cysteine. [More information is available at EcoCyc: EG10192].