catalog number :
MBS033387
products type :
ELISA Kit
products full name :
Bovine Serotonin/5 Hydroxytryptamine ELISA Kit
products short name :
Serotonin/5 Hydroxytryptamine
products gene name :
5-HT
specificity :
No significant cross-reactivity or interference between Bovine 5-HT and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 ng/ml - 500 ng/ml. Sensitivity: 2.0 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 5-HT concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine 5-HT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5-HT and 5-HT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-HT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 5-HT concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine 5-HT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5-HT and 5-HT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-HT in the samples is then determined by comparing the O.D. of the samples to the standard curve.