Human Collagen Type VII ELISA Kit | MyBioSource MBS032407 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Collagen Type VII ELISA Kit

catalog :

MBS032407

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS032407

products type :

ELISA Kit

products full name :

Human Collagen Type VII ELISA Kit

products short name :

Collagen Type VII

other names :

collagen alpha-1(VII) chain; Collagen alpha-1(VII) chain; collagen alpha-1(VII) chain; collagen alpha-1(VII) chain; LC collagen; long-chain collagen; collagen VII, alpha-1 polypeptide; collagen, type VII, alpha 1; Long-chain collagen

products gene name :

COL7

other gene names :

COL7A1; COL7A1; EBD1; EBR1; EBDCT; LC collagen

uniprot entry name :

CO7A1_HUMAN

reactivity :

Human

sequence length :

2944

specificity :

No significant cross-reactivity or interference between Human COL7 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated COL7 concentrations in Human serum, plasma and other body fluids. Using Purified Human COL7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add COL7 and COL7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of COL7 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated COL7 concentrations in Human serum, plasma and other body fluids. Using Purified Human COL7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add COL7 and COL7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of COL7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4502961

ncbi acc num :

NP_000085.1

ncbi gb acc num :

NM_000094.3

uniprot acc num :

Q02388

ncbi mol weight :

295,220 Da

ncbi pathways :

Anchoring Fibril Formation Pathway (730307); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Extracellular Matrix Organization Pathway (576262); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

ncbi summary :

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]

uniprot summary :

COL7A1: the alpha chain of type VII collagen, an extra-cellular basement membrane protein restricted to the zone beneath stratified squamous epithelia. Type VII collagen fibrils are composed of three identical alpha collagen chains. Forms anchoring fibrils, which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Dystrophic epidermolysis bullosa can result from defects in this protein or an autoimmune response made to type VII collagen. Homotrimer. Interacts with MIA3, facilitating its loading into transport carriers and subsequent secretion. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Inhibitor; Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 3p21.1. Cellular Component: extracellular matrix; extracellular space; collagen type VII; endoplasmic reticulum lumen; extracellular region; basement membrane. Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding. Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; epidermis development; cell adhesion. Disease: Transient Bullous Dermolysis Of The Newborn; Epidermolysis Bullosa Dystrophica, Autosomal Dominant; Nail Disorder, Nonsyndromic Congenital, 8; Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Epidermolysis Bullosa Pruriginosa; Epidermolysis Bullosa Dystrophica, Pretibial; Epidermolysis Bullosa With Congenital Localized Absence Of Skin And Deformity Of Nails

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage