ELISA/assay

Human Gastrin-Reliasing Peptide ELISA Kit

MBS032206

48-Strip-Wells

435 USD

ELISA Kit

Human Gastrin-Reliasing Peptide ELISA Kit

Gastrin-Reliasing Peptide

gastrin preproprotein; Gastrin; gastrin; preprogastrin; gastrin; Gastrin component IGastrin-52; G52Big gastrin; Alternative name(s):; Gastrin component II; Gastrin-34; G34Gastrin; Alternative name(s):; Gastrin component III; Gastrin-17

GRP

GAST; GAST; GAS; GAS; G52; G34; G17; G14; G6

GAST_HUMAN

Human

101

No significant cross-reactivity or interference between Human GRP and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 pg/ml - 100 pg/ml. Sensitivity: 1.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GRP concentrations in Human serum, plasma and other body fluids. Using Purified Human GRP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRP and GRP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GRP concentrations in Human serum, plasma and other body fluids. Using Purified Human GRP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRP and GRP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4503923

NP_000796.1

NM_000805.4

P01350

11,394 Da

Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17. [provided by RefSeq, Jul 2008]

gastrin: Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. Belongs to the gastrin/cholecystokinin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: extracellular region. Molecular Function: protein binding; hormone activity. Biological Process: G-protein coupled receptor protein signaling pathway; signal transduction

48-Strip-Wells

435 USD

96-Strip-Wells

600