Human Talin ELISA Kit | MyBioSource MBS032107 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Talin ELISA Kit

catalog :

MBS032107

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS032107

products type :

ELISA Kit

products full name :

Human Talin ELISA Kit

products short name :

Talin

other names :

talin; Talin-1; talin-1; talin 1

products gene name :

TLN

other gene names :

TLN1; TLN1; TLN; ILWEQ; KIAA1027; TLN

uniprot entry name :

TLN1_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human TLN and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TLN concentrations in Human serum, plasma and other body fluids. Using Purified Human TLN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TLN and TLN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TLN in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TLN concentrations in Human serum, plasma and other body fluids. Using Purified Human TLN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TLN and TLN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TLN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6682361

ncbi acc num :

AAF23322.1

uniprot acc num :

Q9Y490

ncbi mol weight :

269,767 Da

ncbi pathways :

Activation Of Chaperone Genes By XBP1(S) Pathway (530771); Activation Of Chaperones By IRE1alpha Pathway (105906); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); EGF Receptor (ErbB1) Signaling Pathway (137970); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); GRB2:SOS Provides Linkage To MAPK Signaling For Intergrins Pathway (106055); HTLV-I Infection Pathway (373901)

ncbi summary :

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]

uniprot summary :

Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. Protein type: Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: ruffle; cell surface; focal adhesion; cytoplasm; plasma membrane; extracellular region; microtubule organizing center; intercellular junction; cytosol. Molecular Function: integrin binding; actin filament binding; LIM domain binding; protein binding; structural constituent of cytoskeleton; insulin receptor binding; vinculin binding. Biological Process: intercellular junction assembly; platelet activation; axon guidance; unfolded protein response, activation of signaling protein activity; platelet degranulation; cellular protein metabolic process; muscle contraction; unfolded protein response; cytoskeletal anchoring; cell-substrate junction assembly; cortical actin cytoskeleton organization and biogenesis; blood coagulation; cell motility

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage