Rabbit Lactoferrin ELISA Kit | MyBioSource MBS032049 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit Lactoferrin ELISA Kit

catalog :

MBS032049

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS032049

products type :

ELISA Kit

products full name :

Rabbit Lactoferrin ELISA Kit

products short name :

Lactoferrin

other names :

lactoferrin; Lactotransferrin; lactotransferrin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12; lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin

products gene name :

other gene names :

LTF; LTF; LF; HLF2; GIG12; GIG12; LF; Lactoferrin; Lfcin-H

uniprot entry name :

TRFL_HUMAN

reactivity :

Rabbit

specificity :

No significant cross-reactivity or interference between Rabbit LF and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 mug/ml - 1000 mug/ml. Sensitivity: 5.0 mug/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LF concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit LF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LF and LF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LF concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit LF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LF and LF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

2104522

ncbi acc num :

AAB57795.1

uniprot acc num :

P02788

ncbi mol weight :

78,182 Da

ncbi pathways :

Amyloids Pathway (366238); Disease Pathway (530764); Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway (645267); Mtb Iron Assimilation By Chelation Pathway (645273); Phagosomal Maturation (early Endosomal Stage) Pathway (645268); Response Of Mtb To Phagocytosis Pathway (645269)

ncbi summary :

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

uniprot summary :

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter. Protein type: EC 3.4.21.-; Secreted, signal peptide; Secreted; Protease. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule. Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; ferric iron binding; DNA binding; iron ion binding; serine-type endopeptidase activity. Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; activation of NF-kappaB transcription factor; regulation of cytokine production; retinal homeostasis; positive regulation of osteoblast differentiation; negative regulation of viral genome replication; innate immune response in mucosa; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; iron assimilation by chelation and transport; negative regulation of ATPase activity; negative regulation of viral reproduction; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage