catalog number :
MBS032029
products type :
ELISA Kit
products full name :
Human Solube Receptor For Advanced Glycation End Products ELISA Kit
products short name :
Solube Receptor For Advanced Glycation End Products
other names :
Sequence 33 from patent US 8043616
products gene name :
SRAGE
specificity :
No significant cross-reactivity or interference between Human SRAGE and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SRAGE concentrations in Human serum, plasma and other body fluids. Using Purified Human SRAGE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SRAGE and SRAGE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SRAGE in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SRAGE concentrations in Human serum, plasma and other body fluids. Using Purified Human SRAGE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SRAGE and SRAGE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SRAGE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AER70450.1