Mouse Neurocan ELISA Kit | MyBioSource MBS031908 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Neurocan ELISA Kit

catalog :

MBS031908

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS031908

products type :

ELISA Kit

products full name :

Mouse Neurocan ELISA Kit

products short name :

Neurocan

other names :

neurocan core protein; Neurocan core protein; neurocan core protein; neurocan proteoglycan; chondroitin sulfate proteoglycan 3 (neurocan); neurocan; Chondroitin sulfate proteoglycan 3

products gene name :

NCAN

other gene names :

NCAN; NCAN; CSPG3; CSPG3; NEUR

uniprot entry name :

NCAN_HUMAN

reactivity :

Mouse

sequence length :

1321

specificity :

No significant cross-reactivity or interference between Mouse NCAN and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NCAN concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse NCAN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NCAN and NCAN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NCAN concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse NCAN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NCAN and NCAN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

118600983

ncbi acc num :

NP_004377.2

ncbi gb acc num :

NM_004386.2

uniprot acc num :

O14594

ncbi mol weight :

143,093 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Axon Guidance Pathway (105688); CS/DS Degradation Pathway (645311); Chondroitin Sulfate Biosynthesis Pathway (645309); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Biosynthesis Pathway (645310); Developmental Biology Pathway (477129); Disease Pathway (530764); ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM, Jul 2002]

uniprot summary :

CSPG3: May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. Belongs to the aggrecan/versican proteoglycan family. Protein type: Secreted, signal peptide; Cell adhesion; Secreted. Chromosomal Location of Human Ortholog: 19p12. Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region. Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding. Biological Process: axon guidance; extracellular matrix organization and biogenesis; central nervous system development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; pathogenesis; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; chondroitin sulfate catabolic process; carbohydrate metabolic process; regulation of synapse structural plasticity; cell adhesion; skeletal development

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage