Mouse Activated Protein C ELISA Kit | MyBioSource MBS031839 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Activated Protein C ELISA Kit

catalog :

MBS031839

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS031839

products type :

ELISA Kit

products full name :

Mouse Activated Protein C ELISA Kit

products short name :

Activated Protein C

other names :

adenomatous polyposis coli protein isoform b; Adenomatous polyposis coli protein; adenomatous polyposis coli protein; deleted in polyposis 2.5; adenomatosis polyposis coli tumor suppressor; protein phosphatase 1, regulatory subunit 46; adenomatous polyposis coli; Deleted in polyposis 2.5

products gene name :

APC

other gene names :

APC; APC; GS; DP2; DP3; BTPS2; DP2.5; PPP1R46; DP2.5; Protein APC

uniprot entry name :

APC_HUMAN

reactivity :

Mouse

sequence length :

2843

specificity :

No significant cross-reactivity or interference between Mouse APC and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 62.5 ng/ml - 2000 ng/ml. Sensitivity: 10 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated APC concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse APC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APC and APC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APC in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated APC concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse APC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APC and APC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

53759122

ncbi acc num :

NP_000029.2

ncbi gb acc num :

NM_000038.5

uniprot acc num :

P25054

ncbi mol weight :

311,646 Da

ncbi pathways :

AMER1 Mutants Destabilize The Destruction Complex Pathway (1016198); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1016193); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1016194); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1016197); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1016195); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); BDNF Signaling Pathway (712093); Basal Cell Carcinoma Pathway (83113)

ncbi summary :

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]

uniprot summary :

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described. Protein type: Motility/polarity/chemotaxis; Tumor suppressor. Chromosomal Location of Human Ortholog: 5q21-q22. Cellular Component: nucleoplasm; kinetochore; centrosome; tight junction; cell-cell adherens junction; lamellipodium; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus; beta-catenin destruction complex; cytosol; lateral plasma membrane. Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; microtubule binding; gamma-catenin binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding. Biological Process: positive regulation of cell adhesion; apoptosis; positive regulation of apoptosis; somatic stem cell maintenance; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; muscle maintenance; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of osteoclast differentiation; hair follicle development; cytokinesis after mitosis; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; cell cycle arrest; kidney development; cell adhesion; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; negative regulation of odontogenesis; regulation of attachment of spindle microtubules to kinetochore; cell migration; thymus development; regulation of osteoblast differentiation; negative regulation of microtubule depolymerization; negative regulation of MAPKKK cascade; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; axonogenesis; dorsal/ventral pattern formation; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration. Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage