ELISA/assay

Human Bcl 2 Antagonist/Killer 1 ELISA Kit

MBS031693

48-Strip-Wells

435 USD

ELISA Kit

Human Bcl 2 Antagonist/Killer 1 ELISA Kit

Bcl 2 Antagonist/Killer 1

bcl-2 homologous antagonist/killer; Bcl-2 homologous antagonist/killer; bcl-2 homologous antagonist/killer; bcl2-L-7; BCL2-like 7 protein; bcl-2-like protein 7; apoptosis regulator BAK; pro-apoptotic protein BAK; BCL2-antagonist/killer 1; Apoptosis regulator BAK; Bcl-2-like protein 7

BAK1

BAK1; BAK1; BAK; CDN1; BCL2L7; BAK-LIKE; BAK; BCL2L7; CDN1; Bcl2-L-7

BAK_HUMAN

Human

211

No significant cross-reactivity or interference between Human BAK1 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated BAK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human BAK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BAK1 and BAK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BAK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated BAK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human BAK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BAK1 and BAK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BAK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4502363

NP_001179.1

NM_001188.3

Q16611

23,409 Da

Activation And Oligomerization Of BAK Protein Pathway (105666); Apoptosis Pathway (198797); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822); DNA Damage Response (only ATM Dependent) Pathway (198827); Direct P53 Effectors Pathway (137939); Integrated Breast Cancer Pathway (219801); Intrinsic Pathway For Apoptosis (105656); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928)

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]

BAK1: In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. Interacts with BCL2A1. Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2, E1B 19k protein, and BCL2L1 isoform Bcl-X(L). Interacts with myxoma virus protein M11L. Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. Belongs to the Bcl-2 family. Protein type: Endoplasmic reticulum; Membrane protein, integral; Apoptosis; Mitochondrial. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: pore complex; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytosol; integral to mitochondrial outer membrane. Molecular Function: BH domain binding; identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; chaperone binding; metal ion binding. Biological Process: response to fungus; regulation of protein heterodimerization activity; positive regulation of proteolysis; regulation of cell cycle; apoptosis; positive regulation of apoptosis; myeloid cell homeostasis; negative regulation of peptidyl-serine phosphorylation; B cell apoptosis; response to organic cyclic substance; negative regulation of cell proliferation; regulation of mitochondrial membrane potential; B cell homeostasis; response to gamma radiation; establishment and/or maintenance of transmembrane electrochemical gradient; B cell negative selection; aging; response to drug; organ regeneration; release of cytochrome c from mitochondria; response to mycotoxin; mitochondrial fusion; regulation of protein homodimerization activity; vagina development; limb morphogenesis; endocrine pancreas development; response to UV-C; cell proliferation; endoplasmic reticulum calcium ion homeostasis; unfolded protein response, activation of signaling protein activity; response to ethanol; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; brain development; regulation of mitochondrial membrane permeability; caspase activation via cytochrome c; post-embryonic camera-type eye morphogenesis

48-Strip-Wells

435 USD

96-Strip-Wells

600