catalog number :
MBS031591
products type :
ELISA Kit
products full name :
Porcine Heat Shock Protein 70 ELISA Kit
products short name :
Heat Shock Protein 70
products gene name :
HSP70
specificity :
No significant cross-reactivity or interference between Porcine HSP70 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HSP70 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine HSP70 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HSP70 and HSP70 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HSP70 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HSP70 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine HSP70 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HSP70 and HSP70 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HSP70 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AAW52766.1