catalog number :
MBS031145
products type :
ELISA Kit
products full name :
Porcine Macrophage Migration Inhibitory Factor ELISA Kit
products short name :
Macrophage Migration Inhibitory Factor
other names :
macrophage migration inhibitory factor; Macrophage migration inhibitory factor; macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase
other gene names :
MIF; MIF; GIF; GLIF; MMIF; GLIF; MMIF; MIF; GIF
uniprot entry name :
MIF_HUMAN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Undiluted original Porcine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of MIF (hereafter termed "analyte") in undiluted original Porcine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_002406.1
ncbi gb acc num :
NM_002415.1
ncbi mol weight :
12,476 Da
ncbi pathways :
Adipogenesis Pathway (198832); Phenylalanine Metabolism Pathway (82960); Phenylalanine Metabolism Pathway (327); Spinal Cord Injury Pathway (739007); Tyrosine Metabolism Pathway (82959); Tyrosine Metabolism Pathway (325)
ncbi summary :
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
uniprot summary :
MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase. Protein type: EC 5.3.3.12; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine; Cell development/differentiation; EC 5.3.2.1; Apoptosis; Isomerase; Cytokine. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle. Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; chemoattractant activity; receptor binding. Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; carboxylic acid metabolic process; positive regulation of lipopolysaccharide-mediated signaling pathway; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of MAP kinase activity; cell proliferation; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of fibroblast proliferation; negative regulation of myeloid cell apoptosis; cell surface receptor linked signal transduction; negative regulation of mature B cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; positive regulation of cytokine secretion; negative regulation of apoptosis. Disease: Rheumatoid Arthritis, Systemic Juvenile