Human Creatine Phosphate ELISA Kit | MyBioSource MBS031083 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Creatine Phosphate ELISA Kit

catalog :

MBS031083

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS031083

products type :

ELISA Kit

products full name :

Human Creatine Phosphate ELISA Kit

products short name :

Creatine Phosphate

other names :

Creatine kinase S-type, mitochondrial; Creatine kinase S-type, mitochondrial; creatine kinase S-type, mitochondrial; S-MtCK; mib-CK; basic-type mitochondrial creatine kinase; sarcomeric mitochondrial creatine kinase; creatine kinase, mitochondrial 2 (sarcomeric); Basic-type mitochondrial creatine kinase; Mib-CK; Sarcomeric mitochondrial creatine kinase

products gene name :

other gene names :

CKMT2; CKMT2; SMTCK; Mib-CK; S-MtCK

uniprot entry name :

KCRS_HUMAN

reactivity :

Human

sequence length :

419

specificity :

No significant cross-reactivity or interference between Human CP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 mmol/L - 1000 mmol/L. Sensitivity: 5.0 mmol/L.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CP concentrations in Human serum, plasma and other body fluids. Using Purified Human CP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CP and CP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CP concentrations in Human serum, plasma and other body fluids. Using Purified Human CP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CP and CP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

116242603

ncbi acc num :

P17540.2

uniprot acc num :

P17540

ncbi mol weight :

47,504 Da

ncbi pathways :

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (106192); Creatine Pathway (413359); Creatine Pathway (468234); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Creatine-phosphate Biosynthesis Pathway (545319); Creatine-phosphate Biosynthesis Pathway (138632)

ncbi summary :

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

CKMT2: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Mitochondrial; Kinase, other. Chromosomal Location of Human Ortholog: 5q13.3. Cellular Component: mitochondrion; mitochondrial inner membrane. Molecular Function: creatine kinase activity; ATP binding. Biological Process: muscle contraction; phosphorylation; creatine metabolic process

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage