ELISA/assay

Human Glucose Regulated Protein 78 ELISA Kit

MBS031039

48-Strip-Wells

435 USD

ELISA Kit

Human Glucose Regulated Protein 78 ELISA Kit

Glucose Regulated Protein 78

GRP78, partial; 78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein

GRP78

HSPA5; HSPA5; BIP; MIF2; GRP78; GRP78; GRP-78; BiP

GRP78_HUMAN

Human

No significant cross-reactivity or interference between Human GRP78 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 50 mug/ml - 1600 mug/ml. Sensitivity: 10 mug/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GRP78 concentrations in Human serum, plasma and other body fluids. Using Purified Human GRP78 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRP78 and GRP78 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRP78 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GRP78 concentrations in Human serum, plasma and other body fluids. Using Purified Human GRP78 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRP78 and GRP78 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRP78 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

386758

AAA52614.1

P11021

72,333 Da

Activation Of Chaperone Genes By ATF6-alpha Pathway (530770); Activation Of Chaperones By ATF6-alpha Pathway (105905); Activation Of Chaperones By IRE1alpha Pathway (105906); Adaptive Immune System Pathway (366160); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Hemostasis Pathway (106028); Immune System Pathway (106386)

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Protein type: Chaperone; Heat shock protein. Chromosomal Location of Human Ortholog: 9q33.3. Cellular Component: signalosome; endoplasmic reticulum membrane; smooth endoplasmic reticulum; focal adhesion; cell surface; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; plasma membrane; melanosome; integral to endoplasmic reticulum membrane; midbody; nucleus. Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; ATPase activity; ribosome binding; calcium ion binding; misfolded protein binding; glycoprotein binding; ATP binding. Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; substantia nigra development; unfolded protein response, activation of signaling protein activity; platelet degranulation; cellular protein metabolic process; positive regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; ER overload response; positive regulation of cell migration; negative regulation of apoptosis

48-Strip-Wells

435 USD

96-Strip-Wells

600