ELISA/assay

Human Mammalian Target of Rapamycin ELISA Kit

MBS030902

48-Strip-Wells

435 USD

ELISA Kit

Human Mammalian Target of Rapamycin ELISA Kit

Mammalian Target of Rapamycin

mTOR, partial

MTOR

Human

No significant cross-reactivity or interference between Human MTOR and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MTOR concentrations in Human serum, plasma and other body fluids. Using Purified Human MTOR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MTOR and MTOR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MTOR in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MTOR concentrations in Human serum, plasma and other body fluids. Using Purified Human MTOR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MTOR and MTOR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MTOR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

433687128

AGB51120.1

48-Strip-Wells

435 USD

96-Strip-Wells

600