ELISA/assay

Human Adrenocorticotropic Hormone ELISA Kit

MBS030317

96 Strip Wells

600 USD

ELISA Kit

Human Adrenocorticotropic Hormone ELISA Kit

Adrenocorticotropic Hormone

ACTH

ACTH

Human

No significant cross-reactivity or interference between Human ACTH and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ACTH concentrations in Human serum, plasma and other body fluids. Using Purified Human ACTH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ACTH and ACTH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ACTH in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 200 pg/ml - 6.25 pg/ml.

Sensitivity: The sensitivity of this kit is 1.0 pg/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ACTH concentrations in Human serum, plasma and other body fluids. Using Purified Human ACTH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ACTH and ACTH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ACTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

490074

CAA00890.1

96 Strip Wells

600 USD