Mouse Hepcidin ELISA Kit | MyBioSource MBS030067 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Hepcidin ELISA Kit

catalog :

MBS030067

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS030067

products type :

ELISA Kit

products full name :

Mouse Hepcidin ELISA Kit

products short name :

Hepcidin

other names :

hepcidin preproprotein; Hepcidin; hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1; hepcidin antimicrobial peptide; Liver-expressed antimicrobial peptide 1; LEAP-1; Putative liver tumor regressor

products gene name :

HEPC

other gene names :

HAMP; HAMP; HEPC; PLTR; HFE2B; LEAP1; HEPC; LEAP1; LEAP-1; PLTR; Hepc25; Hepc20

uniprot entry name :

HEPC_HUMAN

reactivity :

Mouse

specificity :

No significant cross-reactivity or interference between Mouse HEPC and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 1.56 ng/ml - 50 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HEPC concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse HEPC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HEPC and HEPC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HEPC in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HEPC concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse HEPC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HEPC and HEPC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HEPC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

10863973

ncbi acc num :

NP_066998.1

ncbi gb acc num :

NM_021175.2

uniprot acc num :

P81172

ncbi mol weight :

9,408 Da

ncbi pathways :

Iron Metabolism In Placenta Pathway (672461)

ncbi summary :

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]

uniprot summary :

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: apical cortex; extracellular region. Molecular Function: hormone activity. Biological Process: cellular iron ion homeostasis; killing of cells of another organism; defense response to bacterium; immune response; defense response to fungus. Disease: Hemochromatosis, Type 2b

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage