catalog number :
MBS029643
products type :
ELISA Kit
products full name :
Bovine Glutathione Reductase ELISA Kit
products short name :
Glutathione Reductase
other names :
glutathione reductase; At3g54660; glutathione reductase
other gene names :
GR; ATGR2; EMB2360; glutathione reductase; GR
uniprot entry name :
Q5FV38_ARATH
specificity :
No significant cross-reactivity or interference between Bovine GR and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 U/L - 200 U/L. Sensitivity: 1.0 U/L.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GR concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine GR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GR and GR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GR in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GR concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine GR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GR and GR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_191026.1
ncbi gb acc num :
NM_115323.3
ncbi mol weight :
60,852 Da
ncbi pathways :
Glutathione Metabolism Pathway (4032); Glutathione Metabolism Pathway (343); Metabolism Pathway (825961); Metabolism Of Nucleotides Pathway (826082); Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway (826090)
ncbi summary :
Encodes glutathione reductase that is most likely localized in the chloroplast.
uniprot summary :
Sequence similarities: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. RuleBase RU003691