catalog number :
MBS029639
products type :
ELISA Kit
products full name :
Mouse Allograft Inflammatory Factor 1 ELISA Kit
products short name :
Allograft Inflammatory Factor 1
other names :
AIF1; Allograft inflammatory factor 1; allograft inflammatory factor 1; protein G1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1; allograft inflammatory factor 1; Ionized calcium-binding adapter molecule 1; Protein G1
products gene name :
AIF1
other gene names :
AIF1; AIF1; IBA1; IRT1; AIF-1; IRT-1; G1; IBA1; AIF-1
uniprot entry name :
AIF1_HUMAN
specificity :
No significant cross-reactivity or interference between Mouse AIF1 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.
other info2 :
Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate AIF1 concentrations in Mouse serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
products description :
Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate AIF1 concentrations in Mouse serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications!
ncbi acc num :
CAG46950.1
ncbi mol weight :
16,703 Da
ncbi pathways :
Spinal Cord Injury Pathway (739007)
ncbi summary :
This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
uniprot summary :
AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: perinuclear region of cytoplasm; lamellipodium; cytoplasm; actin filament; perikaryon; cytosol; nucleus; phagocytic cup. Molecular Function: actin filament binding; calcium ion binding. Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; microglial cell activation; Rac protein signal transduction; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; response to axon injury; phagocytosis, engulfment; response to electrical stimulus; inflammatory response; negative regulation of apoptosis