Bovine Granzymes B ELISA Kit | MyBioSource MBS029589 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Granzymes B ELISA Kit

catalog :

MBS029589

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS029589

products type :

ELISA Kit

products full name :

Bovine Granzymes B ELISA Kit

products short name :

Granzymes B

other names :

granzyme B; Granzyme B; granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E

products gene name :

GZMS-B

other gene names :

GZMB; GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

uniprot entry name :

GRAB_HUMAN

reactivity :

Bovine

specificity :

No significant cross-reactivity or interference between Bovine GZMS-B and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 pg/ml - 100 pg/ml. Sensitivity: 1.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GZMS-B concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine GZMS-B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GZMS-B and GZMS-B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GZMS-B in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GZMS-B concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine GZMS-B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GZMS-B and GZMS-B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GZMS-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

221625528

ncbi acc num :

NP_004122.2

ncbi gb acc num :

NM_004131.4

uniprot acc num :

P10144

ncbi mol weight :

27,716 Da

ncbi pathways :

Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway (105657); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Apoptosis Pathway (198797); Apoptosis Pathway (105648); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Caspase Cascade In Apoptosis Pathway (137974); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Graft-versus-host Disease Pathway (83124)

ncbi summary :

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

uniprot summary :

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: Protease; EC 3.4.21.79; Apoptosis. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; immunological synapse; nucleus; cytosol. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; protein processing; immune response; proteolysis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage