Porcine Keratan Sulfate ELISA Kit | MyBioSource MBS029539 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Keratan Sulfate ELISA Kit

catalog :

MBS029539

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS029539

products type :

ELISA Kit

products full name :

Porcine Keratan Sulfate ELISA Kit

products short name :

Keratan Sulfate

other names :

keratan sulfate Gal-6-sulfotransferase; Carbohydrate sulfotransferase 1; carbohydrate sulfotransferase 1; carbohydrate (chondroitin 6/keratan) sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; carbohydrate (keratan sulfate Gal-6) sulfotransferase 1; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase

products gene name :

other gene names :

CHST1; CHST1; C6ST; KSST; GST-1; KS6ST; KSGal6ST; GST-1; KS6ST; KSGal6ST; KSST

uniprot entry name :

CHST1_HUMAN

reactivity :

Porcine

sequence length :

411

specificity :

No significant cross-reactivity or interference between Porcine KS and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 ng/ml - 1000 ng/ml. Sensitivity: 5.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated KS concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine KS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KS and KS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KS in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated KS concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine KS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KS and KS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

2887403

ncbi acc num :

BAA24840.1

uniprot acc num :

O43916

ncbi mol weight :

46,715 Da

ncbi pathways :

Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway (82983); Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway (357); Glycosaminoglycan Metabolism Pathway (645297); Keratan Sulfate Biosynthesis Pathway (645302); Keratan Sulfate/keratin Metabolism Pathway (645301); MPS I - Hurler Syndrome Pathway (685537); MPS II - Hunter Syndrome Pathway (685538); MPS IIIA - Sanfilippo Syndrome A Pathway (685539)

ncbi summary :

This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]

uniprot summary :

CHST1: Sulfotransferase that utilizes 3 -phospho-5 -adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N- acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. Protein type: Membrane protein, integral; Transferase; Glycan Metabolism - keratan sulfate biosynthesis; EC 2.8.2.21. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: Golgi membrane; integral to membrane. Molecular Function: sulfotransferase activity; keratan sulfotransferase activity. Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; galactose metabolic process; pathogenesis; polysaccharide metabolic process; inflammatory response

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage