Mouse Interleukin 35 ELISA Kit | MyBioSource MBS029510 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Interleukin 35 ELISA Kit

catalog :

MBS029510

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS029510

products type :

ELISA Kit

products full name :

Mouse Interleukin 35 ELISA Kit

products short name :

Interleukin 35

other names :

interleukin enhancer-binding factor 3 isoform 5; Interleukin enhancer-binding factor 3; interleukin enhancer-binding factor 3; nuclear factor 90; M phase phosphoprotein 4; nuclear factor associated with dsRNA; interleukin enhancer binding factor 3

products gene name :

IL-35

other gene names :

Ilf3; Ilf3; NF90; NFAR; MBII-26; MPHOSPH4

uniprot entry name :

ILF3_MOUSE

reactivity :

Mouse

sequence length :

908

specificity :

No significant cross-reactivity or interference between Mouse IL-35 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-35 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-35 and IL-35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-35 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-35 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-35 and IL-35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

472339106

ncbi acc num :

NP_001264250.1

ncbi gb acc num :

NM_001277321.1

uniprot acc num :

Q9Z1X4

ncbi mol weight :

96,021 Da

ncbi pathways :

MRNA Processing Pathway (198369)

ncbi summary :

The protein encoded by this gene contains two double-stranded RNA binding domains and functions in the post-transcriptional regulation of gene expression. It is a component of an RNA-protein complex that may be involved in mediating the export of messenger RNAs. Alternative splicing results in multiple transcript variants encoding distinct isoforms. These isoforms are grouped into two categories, NFAR-1 or NFAR-2, based on variation at the C-terminus. [provided by RefSeq, Mar 2013]

uniprot summary :

NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described. Protein type: Transcription factor; DNA-binding; Nucleolus; RNA-binding. Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus. Molecular Function: enzyme binding; DNA binding; RNA binding; double-stranded RNA binding. Biological Process: protein amino acid methylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of transcription, DNA-dependent; defense response to virus; protein amino acid phosphorylation

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage