catalog number :
MBS029230
products type :
ELISA Kit
products full name :
Human Dehydroepiandrosterone Sulfate ELISA Kit
products short name :
Dehydroepiandrosterone Sulfate
other names :
DEHA2E14212p; S-adenosylmethionine synthase
products gene name :
DEHA-S
uniprot entry name :
Q6BPE7_DEBHA
specificity :
No significant cross-reactivity or interference between Human DEHA-S and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 25 ug/ml - 800 ug/ml. Sensitivity: 2.0 ug/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DEHA-S concentrations in Human serum, plasma and other body fluids. Using Purified Human DEHA-S antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEHA-S and DEHA-S antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEHA-S in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DEHA-S concentrations in Human serum, plasma and other body fluids. Using Purified Human DEHA-S antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEHA-S and DEHA-S antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEHA-S in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
CAG88165.1
ncbi mol weight :
42,044 Da
ncbi pathways :
Biosynthesis Of Amino Acids Pathway (790484); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Secondary Metabolites Pathway (149849); Cysteine And Methionine Metabolism Pathway (104518); Cysteine And Methionine Metabolism Pathway (103421); Methionine Salvage Pathway (400681); Methionine Salvage Pathway (468227)
uniprot summary :
Function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP . By similarity. Catalytic activity: ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. Cofactor: Binds 1 potassium ion per subunit . By similarity.Binds 2 divalent ions per subunit. Magnesium or cobalt . By similarity. Pathway: Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. Sequence similarities: Belongs to the AdoMet synthase family. RuleBase RU004462