ELISA/assay

Sheep Defensin Beta 2 ELISA Kit

MBS028880

48-Strip-Wells

435 USD

ELISA Kit

Sheep Defensin Beta 2 ELISA Kit

Defensin Beta 2

Defensin beta 2; Beta-defensin 2; beta-defensin 2; defensin beta 2; Defensin, beta 2

DEFbeta2

Defb2; Defb2; BD-2; BD-2; mBD-2

DEFB2_MOUSE

Sheep

No significant cross-reactivity or interference between Sheep DEF?2 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DEF?2 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep DEF?2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEF?2 and DEF?2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEF?2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DEF?2 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep DEF?2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEF?2 and DEF?2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEF?2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

80478968

AAI09130.1

P82020

7,820 Da

Function: Has bactericidal activity . By similarity. Subcellular location: Secreted . By similarity. Tissue specificity: Kidney, uterus and to a lesser extent in heart. Ref.1. Induction: In tracheal epithelium, by lipopolysaccharide or inflammation. Ref.1. Sequence similarities: Belongs to the beta-defensin family.

48-Strip-Wells

435 USD

96-Strip-Wells

600