Rat Amylin ELISA Kit | MyBioSource MBS028823 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Amylin ELISA Kit

catalog :

MBS028823

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS028823

products type :

ELISA Kit

products full name :

Rat Amylin ELISA Kit

products short name :

Amylin

other names :

amylin, partial; Islet amyloid polypeptide; islet amyloid polypeptide; amylin; insulinoma amyloid peptide; diabetes-associated peptide; Islet amyloid polypeptide (diabetes-associated peptide; amylin); islet amyloid polypeptide; Amylin; Diabetes-associated peptide; DAP; Insulinoma amyloid peptide

products gene name :

AMYLIN

other gene names :

IAPP; IAPP; DAP; IAP; DAP

uniprot entry name :

IAPP_HUMAN

reactivity :

Rat

specificity :

No significant cross-reactivity or interference between Rat AMYLIN and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.25 ?mol/L - 8 ?mol/L. Sensitivity: 0.1 ?mol/L.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated AMYLIN concentrations in Rat serum, plasma and other body fluids. Using Purified Rat AMYLIN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AMYLIN and AMYLIN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AMYLIN in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated AMYLIN concentrations in Rat serum, plasma and other body fluids. Using Purified Rat AMYLIN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AMYLIN and AMYLIN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AMYLIN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

457131

ncbi acc num :

AAA35524.1

uniprot acc num :

P10997

ncbi mol weight :

9,806 Da

ncbi pathways :

Amyloids Pathway (366238); Calcitonin-like Ligand Receptors Pathway (106379); Class B/2 (Secretin Family Receptors) Pathway (106378); Developmental Biology Pathway (477129); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Integrated Pancreatic Cancer Pathway (711360); Maturity Onset Diabetes Of The Young Pathway (83096)

ncbi summary :

Islet, or insulinoma, amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Studies suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. [provided by RefSeq, Apr 2011]

uniprot summary :

IAPP: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Belongs to the calcitonin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: extracellular space; cell soma; extracellular region. Molecular Function: identical protein binding; hormone activity; receptor binding. Biological Process: negative regulation of cell differentiation; cell-cell signaling; apoptosis; eating behavior; signal transduction; sensory perception of pain; negative regulation of bone resorption; endocrine pancreas development

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage