ELISA/assay

Mouse Thrombin-Antithrombin Complex ELISA Kit

MBS028506

48-Strip-Wells

435 USD

ELISA Kit

Mouse Thrombin-Antithrombin Complex ELISA Kit

Thrombin-Antithrombin Complex

Tat; p14; Tat

TAT

tat

Mouse

86

No significant cross-reactivity or interference between Mouse TAT and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TAT concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse TAT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TAT and TAT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TAT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TAT concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse TAT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TAT and TAT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

9629358

NP_057853.1

NC_001802.1

Disease Pathway (530764); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); HIV Transcription Elongation Pathway (106001); Host Interactions Of HIV Factors Pathway (106010); Interactions Of Tat With Host Cellular Proteins Pathway (106018); Late Phase Of HIV Life Cycle Pathway (105995); Pausing And Recovery Of Tat-mediated HIV Elongation Pathway (161055); Signaling Events Mediated By HDAC Class III Pathway (137972)

48-Strip-Wells

435 USD

96-Strip-Wells

600