Canine Atrial Natriuretic Peptide-Converting Enzyme ELISA Kit | MyBioSource MBS028059 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Atrial Natriuretic Peptide-Converting Enzyme ELISA Kit

catalog :

MBS028059

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS028059

products type :

ELISA Kit

products full name :

Canine Atrial Natriuretic Peptide-Converting Enzyme ELISA Kit

products short name :

Atrial Natriuretic Peptide-Converting Enzyme

other names :

atrial natriuretic peptide-converting enzyme isoform 1; Atrial natriuretic peptide-converting enzyme; atrial natriuretic peptide-converting enzyme; pro-ANP-convertase; pro-ANP-converting enzyme; transmembrane protease serine 10; heart-specific serine proteinase ATC2; corin, serine peptidase; Corin; Heart-specific serine proteinase ATC2; Pro-ANP-converting enzyme; Transmembrane protease serine 10

products gene name :

ANPCE

other gene names :

CORIN; CORIN; CRN; ATC2; Lrp4; PEE5; TMPRSS10; CRN; TMPRSS10

uniprot entry name :

CORIN_HUMAN

reactivity :

Canine

specificity :

No significant cross-reactivity or interference between Canine ANP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ANP concentrations in Canine serum, plasma and other body fluids. Using Purified Canine ANP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ANP and ANP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ANP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ANP concentrations in Canine serum, plasma and other body fluids. Using Purified Canine ANP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ANP and ANP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ANP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

29244926

ncbi acc num :

NP_006578.2

ncbi gb acc num :

NM_006587.3

uniprot acc num :

Q9Y5Q5

ncbi mol weight :

116,486 Da

ncbi pathways :

Myometrial Relaxation And Contraction Pathways (198759)

ncbi summary :

This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

uniprot summary :

CORIN: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Defects in CORIN are the cause of pre-eclampsia/eclampsia 5 (PEE5); also known as gestational proteinuric hypertension. Preeclampsia is a pregnancy-associated disease with maternal symptoms but placental origin. Unlike most other human disorders, it impacts 2 individuals, the mother and her child, both of whom can be severely affected. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.21.-; Protease; Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 4p12. Cellular Component: cell surface; integral to plasma membrane; plasma membrane; integral to membrane; extracellular region. Molecular Function: serine-type endopeptidase activity. Biological Process: regulation of blood pressure; peptide hormone processing; female pregnancy; proteolysis; regulation of systemic arterial blood pressure by atrial natriuretic peptide. Disease: Preeclampsia/eclampsia 5

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage