catalog number :
MBS028033
products type :
ELISA Kit
products full name :
Rat Interleukin 1beta ELISA Kit
products short name :
Interleukin 1beta
other names :
interleukin-1 beta; interleukin-1 beta
products gene name :
IL-1beta
other gene names :
IL-1beta
specificity :
No significant cross-reactivity or interference between Rat IL-1beta and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 12.5 pg/ml - 400 pg/ml. Sensitivity: 2.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-1beta concentrations in Rat serum, plasma and other body fluids. Using Purified Rat IL-1beta antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-1beta and IL-1beta antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-1beta in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-1beta concentrations in Rat serum, plasma and other body fluids. Using Purified Rat IL-1beta antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-1beta and IL-1beta antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-1beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_001005149.1
ncbi gb acc num :
NM_001005149.1
ncbi pathways :
African Trypanosomiasis Pathway (194379); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (84508); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Apoptosis Pathway (84472); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795)