Rabbit Cyclic Guanosine Monophosphate ELISA Kit | MyBioSource MBS027623 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit Cyclic Guanosine Monophosphate ELISA Kit

catalog :

MBS027623

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS027623

products type :

ELISA Kit

products full name :

Rabbit Cyclic Guanosine Monophosphate ELISA Kit

products short name :

Cyclic Guanosine Monophosphate

other names :

cGMP-dependent protein kinase 1 isoform 1; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase 1; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I; cGMP-dependent protein kinase I

products gene name :

CGMP

other gene names :

PRKG1; PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha; PRKG1B; PRKGR1A; PRKGR1B; cGK 1; cGK1; cGKI

uniprot entry name :

KGP1_HUMAN

reactivity :

Rabbit

sequence length :

671

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Tissue Homogenate, Urine and other Body Fluids. Detection Range: 3.12 ~ 100 nmol/L.

other info2 :

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rabbit CGMP in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Rabbit CGMP concentration. The concentration of Rabbit CGMP in the specimens is then determined by comparing the O.D. of the specimens to the standard curve. Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rabbit CGMP in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Rabbit CGMP concentration. The concentration of Rabbit CGMP in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.

products description :

Background: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of specimens. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the specimen.

ncbi gi num :

148612818

ncbi acc num :

NP_001091982.1

ncbi gb acc num :

NM_001098512.2

uniprot acc num :

Q13976

ncbi mol weight :

76,364 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Ca2+ Pathway (941399); Circadian Entrainment Pathway (698773); Circadian Entrainment Pathway (699872); Gap Junction Pathway (83072); Gap Junction Pathway (483); Hemostasis Pathway (106028); Immune System Pathway (106386); Long-term Depression Pathway (83086); Long-term Depression Pathway (497)

ncbi summary :

Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]

uniprot summary :

PKG1: an AGC kinase of the PKG family. Type I cGMP-dependent protein kinase; relaxes vascular smooth muscle and inhibits platelet aggregation. Binding of cGMP results in enzyme activation. Two splice variant isoforms have been described. Protein type: EC 2.7.11.12; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; PKG family. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: Golgi apparatus; cytoplasm; plasma membrane; cytosol. Molecular Function: protein binding; cGMP-dependent protein kinase activity; calcium channel regulator activity; cGMP binding; ATP binding. Biological Process: regulation of GTPase activity; relaxation of vascular smooth muscle; cGMP-mediated signaling; forebrain development; dendrite development; neuron migration; actin cytoskeleton organization and biogenesis; signal transduction; blood coagulation; protein amino acid phosphorylation

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage