Rat Myelin Associated Glycoprotein ELISA Kit | MyBioSource MBS027530 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Myelin Associated Glycoprotein ELISA Kit

catalog :

MBS027530

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS027530

products type :

ELISA Kit

products full name :

Rat Myelin Associated Glycoprotein ELISA Kit

products short name :

Myelin Associated Glycoprotein

other names :

myelin-associated glycoprotein isoform a; Myelin-associated glycoprotein; myelin-associated glycoprotein; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A; myelin associated glycoprotein; Siglec-4a

products gene name :

MAG

other gene names :

MAG; MAG; GMA; S-MAG; SIGLEC4A; SIGLEC-4A; GMA

uniprot entry name :

MAG_HUMAN

reactivity :

Rat

specificity :

No significant cross-reactivity or interference between Rat MAG and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MAG concentrations in Rat serum, plasma and other body fluids. Using Purified Rat MAG antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MAG and MAG antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MAG in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MAG concentrations in Rat serum, plasma and other body fluids. Using Purified Rat MAG antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MAG and MAG antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MAG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

11225258

ncbi acc num :

NP_002352.1

ncbi gb acc num :

NM_002361.3

uniprot acc num :

P20916

ncbi mol weight :

69,069 Da

ncbi pathways :

Axonal Growth Inhibition (RHOA Activation) Pathway (106454); Basigin Interactions Pathway (106065); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Glial Cell Differentiation Pathway (698758); Hemostasis Pathway (106028); Signal Transduction Pathway (477114); Signalling By NGF Pathway (106439); Spinal Cord Injury Pathway (739007)

ncbi summary :

The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]

uniprot summary :

MAG: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3- linked sialic acid. Binds to RTN4R. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: paranode region of axon; plasma membrane; integral to membrane. Molecular Function: carbohydrate binding. Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; negative regulation of axonogenesis; regulation of axonogenesis; cell adhesion; blood coagulation; leukocyte migration

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage