ELISA/assay

Horse Glycogen Synthase Kinase 3 Alpha ELISA Kit

MBS027459

48-Strip-Wells

435 USD

ELISA Kit

Horse Glycogen Synthase Kinase 3 Alpha ELISA Kit

Glycogen Synthase Kinase 3 Alpha

glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 alpha; glycogen synthase kinase-3 alpha; GSK-3 alpha; serine/threonine-protein kinase GSK3A; glycogen synthase kinase 3 alpha; Serine/threonine-protein kinase GSK3A

GSK3A

GSK3A; GSK3A; GSK-3 alpha

GSK3A_HUMAN

Horse

No significant cross-reactivity or interference between Horse GSK3A and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 50 pmol/L - 1600 pmol/L. Sensitivity: 10 pmol/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GSK3A concentrations in Horse serum, plasma and other body fluids. Using Purified Horse GSK3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GSK3A and GSK3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GSK3A in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GSK3A concentrations in Horse serum, plasma and other body fluids. Using Purified Horse GSK3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GSK3A and GSK3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GSK3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

49574532

NP_063937.2

NM_019884.2

P49840

50,981 Da

AKT Phosphorylates Targets In The Cytosol Pathway (106475); Activation Of Chaperone Genes By XBP1(S) Pathway (530771); Activation Of Chaperones By IRE1alpha Pathway (105906); Adaptive Immune System Pathway (366160); B Cell Receptor Signaling Pathway (198909); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549)

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418. Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, CMGC; Kinase, protein; EC 2.7.11.26; CMGC group; GSK family; GSK subfamily. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: cytosol; beta-catenin destruction complex. Molecular Function: protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; ATP binding. Biological Process: glycogen metabolic process; proteasomal ubiquitin-dependent protein catabolic process; genetic imprinting; nerve growth factor receptor signaling pathway; negative regulation of insulin receptor signaling pathway; protein amino acid phosphorylation; negative regulation of transferase activity; positive regulation of cAMP biosynthetic process; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; nervous system development; negative regulation of TOR signaling pathway; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; Wnt receptor signaling pathway; unfolded protein response; positive regulation of peptidyl-serine phosphorylation; negative regulation of glucose import; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; regulation of systemic arterial blood pressure; positive regulation of protein catabolic process; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of heart contraction

48-Strip-Wells

435 USD

96-Strip-Wells

600