Canine Resistin ELISA Kit | MyBioSource MBS027419 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Resistin ELISA Kit

catalog :

MBS027419

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS027419

products type :

ELISA Kit

products full name :

Canine Resistin ELISA Kit

products short name :

Resistin

other names :

resistin; Resistin; resistin; resistin delta2; found in inflammatory zone 3; cysteine-rich secreted protein FIZZ3; adipose tissue-specific secretory factor; cysteine-rich secreted protein A12-alpha-like 2; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; resistin; Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3

products gene name :

RESISTIN

other gene names :

RETN; RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1; FIZZ3; HXCP1; RSTN; ADSF

uniprot entry name :

RETN_HUMAN

reactivity :

Canine

specificity :

No significant cross-reactivity or interference between Canine RSN and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RSN concentrations in Canine serum, plasma and other body fluids. Using Purified Canine RSN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RSN and RSN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RSN in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RSN concentrations in Canine serum, plasma and other body fluids. Using Purified Canine RSN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RSN and RSN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RSN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

12584202

ncbi acc num :

AAG59824.1

uniprot acc num :

Q9HD89

ncbi mol weight :

11,419 Da

ncbi pathways :

Adipogenesis Pathway (198832)

ncbi summary :

This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

uniprot summary :

resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular space; nucleus. Molecular Function: hormone activity. Biological Process: fat cell differentiation; response to mechanical stimulus; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; positive regulation of synaptic transmission; response to insulin stimulus; aging. Disease: Diabetes Mellitus, Noninsulin-dependent

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage