catalog number :
MBS027326
products type :
ELISA Kit
products full name :
Bovine 5-Nucleotidase ELISA Kit
products short name :
[5-Nucleotidase]
other names :
[5'-nucleotidase; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; 5' nucleotidase, ecto; Ecto-5'-nucleotidase]
products gene name :
[5NT]
other gene names :
[Nt5e; Nt5e; Nt5; CD73; Nt5; Nte; 5'-NT]
uniprot entry name :
5NTD_RAT
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of 5NT (hereafter termed "analyte") in undiluted original Bovine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_067587.2
ncbi gb acc num :
NM_021576.2
ncbi mol weight :
63,969 Da
ncbi pathways :
Metabolism Pathway (828668); Metabolism Of Nucleotides Pathway (828815); Nicotinate And Nicotinamide Metabolism Pathway (83406); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (828819); Purine Metabolism Pathway (83337); Purine Metabolism Pathway (828816); Purine Metabolism Pathway (307); Pyrimidine Catabolism Pathway (828823); Pyrimidine Metabolism Pathway (83339)
ncbi summary :
may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]
uniprot summary :
NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.3.5; Membrane protein, GPI anchor; Phosphatase (non-protein); Membrane protein, integral; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - pyrimidine. Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane. Molecular Function: 5 -nucleotidase activity; ferrous iron binding; metal ion binding; nucleotide binding. Biological Process: dephosphorylation; negative regulation of inflammatory response; adenosine biosynthetic process; brain development; purine nucleotide biosynthetic process; positive regulation of lipid biosynthetic process; adenosine metabolic process; response to aluminum ion; AMP catabolic process
size4 :
10x96-Strip-Wells