Horse Neutrophil Gelatinase Associated Lipocalin/Lipocalin-2 ELISA Kit | MyBioSource MBS027262 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Horse Neutrophil Gelatinase Associated Lipocalin/Lipocalin-2 ELISA Kit

catalog :

MBS027262

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS027262

products type :

ELISA Kit

products full name :

Horse Neutrophil Gelatinase Associated Lipocalin/Lipocalin-2 ELISA Kit

products short name :

Neutrophil Gelatinase Associated Lipocalin/Lipocalin-2

other names :

neutrophil gelatinase-associated lipocalin; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; lipocalin 2; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25

products gene name :

NGAL/LCN2

other gene names :

LCN2; LCN2; 24p3; MSFI; NGAL; HNL; NGAL; NGAL

uniprot entry name :

NGAL_HUMAN

reactivity :

Horse

specificity :

No significant cross-reactivity or interference between Horse NGAL/LCN2 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NGAL/LCN2 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse NGAL/LCN2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NGAL/LCN2 and NGAL/LCN2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NGAL/LCN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NGAL/LCN2 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse NGAL/LCN2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NGAL/LCN2 and NGAL/LCN2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NGAL/LCN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

38455402

ncbi acc num :

NP_005555.2

ncbi gb acc num :

NM_005564.3

uniprot acc num :

P80188

ncbi mol weight :

22,588 Da

uniprot summary :

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding. Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; siderophore transport; positive regulation of cell projection organization and biogenesis; innate immune response; ion transport; response to herbicide; transmembrane transport

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage