Bovine Mucin-5B ELISA Kit | MyBioSource MBS026918 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Mucin-5B ELISA Kit

catalog :

MBS026918

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS026918

products type :

ELISA Kit

products full name :

Bovine Mucin-5B ELISA Kit

products short name :

Mucin-5B

other names :

mucin-5B; Mucin-5B; mucin-5B; cervical mucin MUC5B; sublingual gland mucin; mucin 5, subtype B, tracheobronchial; high molecular weight salivary mucin MG1; mucin 5B, oligomeric mucus/gel-forming; Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B, tracheobronchial; Sublingual gland mucin

products gene name :

MUC5B

other gene names :

MUC5B; MUC5B; MG1; MUC5; MUC9; MUC-5B; MUC5; MUC-5B

uniprot entry name :

MUC5B_HUMAN

reactivity :

Bovine

specificity :

No significant cross-reactivity or interference between Bovine MUC5B and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MUC5B concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine MUC5B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MUC5B and MUC5B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MUC5B in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MUC5B concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine MUC5B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MUC5B and MUC5B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MUC5B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

301172750

ncbi acc num :

NP_002449.2

ncbi gb acc num :

NM_002458.2

uniprot acc num :

Q9HC84

ncbi mol weight :

596,340 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Termination Of O-glycan Biosynthesis Pathway (530748)

ncbi summary :

This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010]

uniprot summary :

MUC5B: Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. Defects in MUC5B are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. A common polymorphism in the promoter of MUC5B is associated with familial interstitial pneumonia and idiopathic pulmonary fibrosis, suggesting that dysregulated MUC5B expression in the lung may be involved in the pathogenesis of pulmonary fibrosis (PubMed:21506741). Protein type: Cell adhesion; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; Golgi lumen. Molecular Function: protein binding. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; regulation of macrophage activation; defense response to bacterium; post-translational protein modification. Disease: Pulmonary Fibrosis, Idiopathic

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage