catalog number :
MBS026891
products type :
ELISA Kit
products full name :
Human Procollagen Type I C-Terminal Propeptide ELISA Kit
products short name :
Procollagen Type I C-Terminal Propeptide
other names :
Procollagen C-endopeptidase enhancer 1; Procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer 1; astroglial cell transcript 2; procollagen C-proteinase enhancer 1; procollagen C-proteinase enhancer protein; procollagen COOH-terminal proteinase enhancer 1; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer protein; P14; Procollagen COOH-terminal proteinase enhancer 1; PCPE-1; Procollagen C-proteinase enhancer 1; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer
products gene name :
PICP
other gene names :
Pcolce; Pcolce; P14; Astt2; Astt-2; PCPE-1; Pcpe1; PCPE-1
uniprot entry name :
PCOC1_MOUSE
specificity :
No significant cross-reactivity or interference between Human PICP and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 25 ng/ml - 800 ng/ml. Sensitivity: 2.0 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PICP concentrations in Human serum, plasma and other body fluids. Using Purified Human PICP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PICP and PICP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PICP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PICP concentrations in Human serum, plasma and other body fluids. Using Purified Human PICP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PICP and PICP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PICP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi mol weight :
50,168 Da
ncbi pathways :
Collagen Biosynthesis And Modifying Enzymes Pathway (1001017); Collagen Formation Pathway (1001016); Extracellular Matrix Organization Pathway (1001015); MRNA Processing Pathway (198369)
uniprot summary :
PCOLCE: Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. Protein type: Extracellular matrix; Secreted, signal peptide; Secreted. Cellular Component: extracellular matrix; extracellular space; extracellular region. Molecular Function: collagen binding; heparin binding; protease activator activity. Biological Process: proteolysis