Human Apolipoprotein A4 ELISA Kit | MyBioSource MBS026183 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Apolipoprotein A4 ELISA Kit

catalog :

MBS026183

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS026183

products type :

ELISA Kit

products full name :

Human Apolipoprotein A4 ELISA Kit

products short name :

Apolipoprotein A4

other names :

apolipoprotein A-IV; Apolipoprotein A-IV; apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4; apolipoprotein A-IV; Apolipoprotein A4

products gene name :

APOA4

other gene names :

APOA4; APOA4; Apo-AIV; ApoA-IV

uniprot entry name :

APOA4_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human APOA4 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 mug/ml - 100 mug/ml. Sensitivity: 1.0 ug/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated APOA4 concentrations in Human serum, plasma and other body fluids. Using Purified Human APOA4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOA4 and APOA4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated APOA4 concentrations in Human serum, plasma and other body fluids. Using Purified Human APOA4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOA4 and APOA4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

71773110

ncbi acc num :

NP_000473.2

ncbi gb acc num :

NM_000482.3

uniprot acc num :

P06727

ncbi mol weight :

45,399 Da

ncbi pathways :

Amyloids Pathway (366238); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ncbi summary :

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

uniprot summary :

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family. Protein type: Lipid-binding; Secreted, signal peptide; Endoplasmic reticulum; Secreted; Cell adhesion. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol. Molecular Function: lipid transporter activity; antioxidant activity; protein binding; copper ion binding; protein homodimerization activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding. Biological Process: cholesterol metabolic process; positive regulation of fatty acid biosynthetic process; multicellular organismal lipid catabolic process; phototransduction, visible light; removal of superoxide radicals; protein-lipid complex assembly; neurite regeneration; lipid homeostasis; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; leukocyte adhesion; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; innate immune response in mucosa; regulation of cholesterol absorption; response to lipid hydroperoxide; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport; retinoid metabolic process

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage