Human Asymmetric Dimethylaoyoinine ELISA Kit | MyBioSource MBS025713 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Asymmetric Dimethylaoyoinine ELISA Kit

catalog :

MBS025713

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS025713

products type :

ELISA Kit

products full name :

Human Asymmetric Dimethylaoyoinine ELISA Kit

products short name :

Asymmetric Dimethylaoyoinine

other names :

Alanine--glyoxylate aminotransferase 2, mitochondrial; Alanine--glyoxylate aminotransferase 2, mitochondrial; alanine--glyoxylate aminotransferase 2, mitochondrial; beta-ALAAT II; beta-alanine-pyruvate aminotransferase; (R)-3-amino-2-methylpropionate--pyruvate transaminase; alanine--glyoxylate aminotransferase 2; (R)-3-amino-2-methylpropionate--pyruvate transaminase (EC:2.6.1.40); Beta-ALAAT II; Beta-alanine-pyruvate aminotransferase; D-AIBAT

products gene name :

ADMA

other gene names :

AGXT2; AGXT2; AGT2; DAIBAT; AGT2; AGT 2

uniprot entry name :

AGT2_HUMAN

reactivity :

Human

sequence length :

514

specificity :

No significant cross-reactivity or interference between Human ADMA and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 ng/ml - 1000 ng/ml. Sensitivity: 5.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ADMA concentrations in Human serum, plasma and other body fluids. Using Purified Human ADMA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ADMA and ADMA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ADMA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ADMA concentrations in Human serum, plasma and other body fluids. Using Purified Human ADMA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ADMA and ADMA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ADMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

17432913

ncbi acc num :

Q9BYV1.1

uniprot acc num :

Q9BYV1

ncbi mol weight :

57,156 Da

ncbi pathways :

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Glyoxylate Metabolism Pathway (160990); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Catabolism Pathway (106288); Pyrimidine Metabolism Pathway (106281)

ncbi summary :

The protein encoded by this gene is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]

uniprot summary :

AGXT2: is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]. Protein type: Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; EC 2.6.1.40; EC 2.6.1.44. Chromosomal Location of Human Ortholog: 5p13. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: beta-alanine-pyruvate transaminase activity; alanine-glyoxylate transaminase activity; (R)-3-amino-2-methylpropionate-pyruvate transaminase activity; pyridoxal phosphate binding. Biological Process: pyrimidine nucleoside catabolic process; positive regulation of nitric oxide biosynthetic process; glyoxylate catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; glyoxylate metabolic process; glycine biosynthetic process, by transamination of glyoxylate; L-alanine catabolic process, by transamination

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage