ELISA/assay

Porcine Syndecan-1/Cd138 ELISA Kit

MBS025458

48-Strip-Wells

435 USD

ELISA Kit

Porcine Syndecan-1/Cd138 ELISA Kit

Syndecan-1/Cd138

syndecan-1; Syndecan-1; syndecan-1; CD138 antigen; syndecan proteoglycan 1; heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1

SDC1/CD138

SDC1; SDC1; SDC; CD138; SYND1; syndecan; SDC; SYND1

SDC1_HUMAN

Porcine

No significant cross-reactivity or interference between Porcine SDC1/CD138 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 mug/ml - 200 mug/ml. Sensitivity: 1.0 mug/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SDC1/CD138 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine SDC1/CD138 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SDC1/CD138 and SDC1/CD138 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SDC1/CD138 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SDC1/CD138 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine SDC1/CD138 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SDC1/CD138 and SDC1/CD138 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SDC1/CD138 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

55749480

NP_001006947.1

NM_001006946.1

P18827

32,462 Da

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Arf6 Trafficking Events Pathway (137954); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479)

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family. Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p24.1. Cellular Component: lysosomal lumen; protein complex; focal adhesion; cell surface; integral to plasma membrane; Golgi lumen; cytoplasm; plasma membrane; external side of plasma membrane. Molecular Function: protein C-terminus binding; protein binding; glycoprotein binding. Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; myoblast development; response to cAMP; glycosaminoglycan metabolic process; wound healing; striated muscle cell development; response to toxin; response to glucocorticoid stimulus; pathogenesis; lipoprotein metabolic process; Wnt receptor signaling pathway through beta-catenin; Sertoli cell development; chondroitin sulfate metabolic process; odontogenesis; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; response to hydrogen peroxide; ureteric bud development; carbohydrate metabolic process; inflammatory response; retinoid metabolic process; response to calcium ion

48-Strip-Wells

435 USD

96-Strip-Wells

600